Optimization of Real-time Reverse Transcriptase Polymerase Chain Reaction for Detection of Dengue Virus
نویسندگان
چکیده
منابع مشابه
Real-time quantitative reverse transcriptase polymerase chain reaction.
The real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) has become the method of choice for the quantification of specific mRNAs. This method is fast, extremely sensitive, and accurate, requires only very small amounts of input RNA, and is relatively simple to perform. These characteristics have made it the method of choice for minimal residual disease monitoring suc...
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A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecti...
متن کاملA real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus
Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular stomatitis disease in cattle, horses and pigs, as well as sporadic human cases of acute febrile illness. Therefore, diagnosis of VSV infections by reliable laboratory techniques is important to allow a proper case management and implementation of strategies for the containment of virus spread. We show here a sensitive and reproduci...
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Background and Aims: Accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (FMD). In present study, was evaluated real time reverse transcription-polymerase chain reaction (rRT-PCR) assay along with diagnostic routine methods for the detection of all seven serotypes of FMD virus (FMDV), namely O, C, A, SAT1, 2, 3 and Asia 1 in biological sample...
متن کاملبررسی بیان ژن vegf در بیماران مبتلا به سرطان مری با استفاده از روش reverse transcriptase real- time polymerase chain reaction
سرطان مری با حدود 386000 مرگ و میر در سال ، ششمین عامل مرگ ناشی از سرطان در جهان است. vegf ، یکی از مهمترین تنظیم کننده های اختصاصی رگ زایی است.ژن vegf در انواع مختلف سرطان مری جهش یافته است. این ژن در مقابل ایجاد جهش در dna وکمبود اکسیژن در تومور فعال شده و تومور به واسطه بیان زیاد این ژن ، رگزایی را آغاز کرده و کمبود اکسیژن خود را از دریافت اکسیژن از خون دریافت کرده و در نهایت متاستاز تومور ر...
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ژورنال
عنوان ژورنال: Journal of Advances in Microbiology
سال: 2020
ISSN: 2456-7116
DOI: 10.9734/jamb/2020/v20i830269